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Calcium-flux assay on the FDSS/µCELL System

Calcium ions play a critical role in the electrophysiology of the heart and regulate the activities of motor proteins necessary for cardiac contraction. Direct acute drug effects on both electrophysiology and contractility can be detected by monitoring Ca2+ transients, making it a valuable tool in drug discovery and safety pharmacology assessment. The FDSS/µCell instrument (Hamamatsu) is a kinetic plate reader with an integrated dispensing head and imaging-based detector. Upon treatment with a calcium fluorescent dye, real-time changes in fluorescent calcium transients can be measured in hiPSC-derived cardiomyocytes at a high-throughput (96- and 384-well plate formats).

User guide

hiPSC-derived cardiomyocytes optimized for use on the FDSS/µCELL System

Human iPSC-derived cardiomyocytes utilization with the FDSS/µCell instrument has been optimized. Combining these technologies enables the evaluation of potential cardiotoxic effects of compounds at high-throughput (up to 384 wells), thereby supporting your decision making in the safety of drug candidates at an early stage of drug development.

You can find a step-by-step description of the analysis of fluorescent dye-based calcium transients of our hiPSC-derived cardiomyocytes using the FDSS/µCell System in our User Guide:

Application

Drug safety assessment using hiPSC-derived cardiomyocytes in combination with the FDSS/µCELL System

Case studies were performed to assess the acute effects of well-known cardioactive compounds in Pluricyte® Cardiomyocytes calcium transients using the FDSS/µCELL kinetic plate reader. Acute effects of cardioactive compounds were determined to analyze various parameters, including peak frequency, peak amplitudes, peak slopes, and average peak width duration.

You can find the complete study in our application note: