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MEA measurements of neurons using the Maestro™ MEA System

Measuring the extracellular action potentials of hiPSC-derived neurons can be used to detect drug-induced effects on the electrophysiology of the neurons. Action potentials can be obtained noninvasively using microelectrode array (MEA) technology.

User guide

Peri.4U® and CNS.4U® neurons are optimized for use on the Maestro™ MEA System

The use of Peri.4U® and CNS.4U® neurons in combination with the Maestro™ MEA system has been optimized extensively. Combining the neurons with this technology in 48-well plate format enables detection and detailed analysis of spike activity and spike pattern affected and modulated by test compounds.

Peri.4U® and CNS.4U® neurons in combination with the Maestro™ MEA system provide a highly relevant in vitro assay platform to study the neurotoxicological profile of compounds during drug development.

You can find our recommendations on how to combine our hiPSC-derived neurons with the Maestro™ MEA system on 48- and 96-well plate formats for electrophysiology measurements in the following User Guides:

Application

Assessment of seizure-liability using hiPSC-derived central nervous neurons in combination with the Maestro™ MEA system

A pilot study was performed to assess the effects of seizure-related compounds on the electrophysiology of CNS.4U® neurons using the Maestro™ MEA system.

The CNS.4U® neurons responded to the compounds tested by specific changes of their spike activity and spike pattern as expected. Below, an example is shown of the effect of the reference compound, picrotoxin, in CNS.4U® neurons.

Picrotoxin improved the structure level in the spontaneous activity of CNS.4U® neurons

MEA technology was used to assess effects of seizure-inducing compound picrotoxin. Raster plots illustrate the distribution of action potential detected in 9 electrodes per well over time. Picrotoxin did significantly improve the network burst coordination in CNS.4U® neurons.

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