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MEA on the Multiwell-MEA-System

Measuring the extracellular voltage of cardiomyocytes can be used to detect drug-induced effects on the behavior of the cells. These extracellular field potentials can be obtained noninvasively without disruption of the membrane or the need for potentially toxic dyes by culturing cardiomyocytes on a microelectrode array (MEA). This enables long-term MEA recordings over time on the same culture. The Multiwell-MEA-System (Multi Channel Systems) enables these field potential recordings in a 96-well plate format.

Application

Cardiac safety assessment of cardiomyocytes on the Multiwell-MEA-System

Case studies were performed to assess the effects of cardioactive compounds on the electrophysiology of Pluricyte® Cardiomyocytes using the Multiwell-MEA-System (Multi Channel Systems). The effects of cardioactive reference compounds on the field potential duration, beat period, depolarization peak and conduction velocity are shown here.

Dofetilide induced a concentration-dependent increase of the field potential duration in Pluricyte® Cardiomyocytes

Dofetilide is a hERG channel blocker that is expected to prolong the repolarization, resulting in prolonged field potential duration (FPD) and ultimately proarrhythmic events. The induced concentration-dependent increase of the FPD in Pluricyte® Cardiomyocytes is shown here (first panel). At 100 nM, arrhythmias were observed (second panel).

 

You can find the complete study in our application note: