47 Rue Adrienne Bolland
Calcium-sensitive dyes are absorbed into the cell’s cytoplasm during incubation and exhibit changes in fluorescence upon calcium binding. Calcium sensitive dyes are a sensitive and rapid indicator of calcium changes.
Genetically encoded calcium indicators, like GCaMP, are protein-based indicators introduced into the cells by transduction. The main advantage of using GECI is that they enable real time calcium measurements which is of interest studying long term toxic compound effects.
Drug-induced changes in blood pressure are an unwanted but common effect of novel compounds. Calcium transient measurements on hiPSC-derived vascular smooth muscle cells enables detection of vasodilators and vasoconstrictors.
A concentration-dependent response to the vasoconstrictor angiotensin-II.