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Endothelial permeability

Since disruption of the endothelial layer is an important feature for many diseases, early screening of endothelial permeability is essential in the drug discovery process. Vascular leakage should be taken into account as well, being a dose-limiting adverse effect for many therapeutics under development. Our experts use a high-throughput, real-time and label-free technology to assess endothelial permeability, which allows in-depth analyses of vascular barrier function in health and disease for your drug discovery projects.

 

Benefits

Real-time monitoring
of barrier function disruption and recovery

Multiplexing assay
Noninvasive nature of the readout allows for multiple experiments

High quality results
Based on 10+ years of Ncardia experience

Description

Impedance assay

Our scientists assess endothelial permeability through impedance assays. This allows for monitoring of a wide range of physiological and pathophysiological mechanisms for drug discovery, to assess both compound safety and efficacy. Compared to other endothelial permeability assay types, like transwells, electrical impedance is more sensitive, and enables time-lapse tracking.

Our advanced data analysis capabilities enable calculation of time course changes in cell permeability, cell-matrix interaction and the cell membrane capacitance. Such analyses enable discrimination between necrosis and apoptosis and between subtle loss of cell-cell contacts or acute cell death.

Assay Capabilities

ECIS system xCelligence RTCA CardioECR systemNanion CardioExcyte 96 system

Interested?

Would you like to know more about our Endothelial Permeability service?

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Related

Manufacturing Service 

Vascular endothelial cells

Poster 

Simultaneous measurement of contractility, electrophysiology and troponin I secretion to determine cardiotoxicity