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hiPSC-derived cardiomyocytes are cultured on Cardio.Force plates featuring a flexible culture substrate composed of a ultra-thin silicone membrane. Together with these culture plates the custom made assay system allows for measurements and quantification of the contraction-induced deflection of the silicone membranes. This is facilitated by a combination of capacitive distance sensors and a pressure sensors beneath the membrane of each well. All measurements are performed in a cell culture incubator at 37°C and 5% CO2.
Inotropic compounds change the force of heart muscle contraction. Positive inotropes, that strengthen the contraction, can be used for patients with cardiomyopathy. Negative inotropes, that weakens the contraction, are used for hypertension, arrhythmia, and angina.
Assessment of hiPSC-derived cardiomyocytes on the Cardio.Force, demonstrates the positive inotrope effect of isoproterenol, and the negative inotrope effect of verapamil.