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High Content Imaging can be applied to many steps in the drug discovery process. It can be used as a primary high-throughput screening tool to analyze potential leads, optimizing your hit identification strategies. As your drug targets or lead compounds progress into lead optimization, we can help you visualize and quantify phenotypic changes in more complex cellular systems to predict success in vivo.
Hypertrophic cardiomyopathy (HCM) is an autosomal dominant disease of the cardiac sarcomere. In this case study, we created an HCM disease model from iPSC-derived cardiomyocytes. We measured BNP expression through overnight ET-1 exposure by immunofluorescence microscopy (see visual below).
After successful assay validation, we screened ~5000 data points including >1800 approved drugs, and a thousand further compounds with known mechanism of action. Hits were confirmed via three distinct assays; NT-proBNP AlphaLISA assay, AlphaLISATruHits assay (deselect false positives), and intracellular expression assessed by a HCI secondary assay.
Click here to read the full case study: Hypertrophic cardiomyopathy
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