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High Content Imaging

Through our high content imaging (HCI) service, we link the effects of your compounds to physiological and morphological cellular changes, delivering valuable insights for studying safety or efficacy profiles. Implementing HCI in an early stage of your drug discovery research is an effective way to increase the quality and efficiency. At Ncardia, we have the equipment and expertise to provide you with high quality screening results throughout your research.


Benefits

Efficient hit identification
By analyzing multiple targets simultaneously

Accurate prediction of success in vivo
Through assessment of complex cellular structures

High quality results
Based on 10+ years of Ncardia experience

Description

High Content Imaging can be applied to many steps in the drug discovery process. It can be used as a primary high-throughput screening tool to analyze potential leads, optimizing your hit identification strategies. As your drug targets or lead compounds progress into lead optimization, we can help you visualize and quantify phenotypic changes in more complex cellular systems to predict success in vivo.

Case Study

Hypertrophic cardiomyopathy

Hypertrophic cardiomyopathy (HCM) is an autosomal dominant disease of the cardiac sarcomere. In this case study, we created an HCM disease model from iPSC-derived cardiomyocytes. We measured BNP expression through overnight ET-1 exposure by immunofluorescence microscopy (see visual below).

After successful assay validation, we screened ~5000 data points including >1800 approved drugs, and a thousand further compounds with known mechanism of action. Hits were confirmed via three distinct assays; NT-proBNP AlphaLISA assay, AlphaLISATruHits assay (deselect false positives), and intracellular expression assessed by a HCI secondary assay.

Click here to read the full case study: Hypertrophic cardiomyopathy

Measurements of BNP expression through overnight ET-1 exposure by immunofluorescence microscopy

Assay Capabilities

ImageXpress Micro Confocal system

Interested?

Would you like to know more about our High Content Imaging assay service?

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Related

Case study 

Phenotypic screen of hypertrophic cardiomyopathy

Cell model 

hiPSC-derived central nervous system neuron cells and astrocytes