47 Rue Adrienne Bolland
Neurite growth is essential to the formation of neural networks and nerve regeneration. Studying these networks provides useful information to drug discovery projects on neurodegeneration as well as neurotoxicity. Human iPSC-derived cortical neurons are a suitable model to study neurite outgrowth in HCI.
Human iPSC-derived cortical neurons were stained for Tubulin beta 3 (green) and nuclei (blue). Cell bodies were identified and a binary mask of the neurite network was obtained, hence allowing to calculate total neurite length for the respective field of view. Images were acquired with a 20x objective to resolve individual neurites.