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Metabolism assays

Abnormal metabolic functioning is involved in many human diseases including atherosclerosis, cardiomyopathies, ischemia reperfusion injury, and neurodegenerative disorders. Ncardia offers a range of metabolism assays that monitor the metabolic profile, which is essential to assess the impact of candidate compounds that have either a protective or diminishing effect on cell metabolism.

This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 737978.


Benefits

Multiplexing assay
Assess multiple targets simultaneously

Tailored to your project needs
Wide range of assays to test metabolic activities

High quality results
Based on 10+ years of Ncardia experience

Description

Oxygen Consumption Rate (OCR) assay

OCR is a very good indicator of mitochondrial function. The oxidative phosphorylation capabilities of cells can be evaluated with this assay.


Extracellular Acidification Rate (ECAR) assay

ECAR is a good measure of the glycolytic activity in cells. By recording lactate production via the acidification rate, this assay can monitor the glycolytic activity of cells.


Intracellular Oxygen Concentration Assay

The Intracellular Oxygen Concentration Assay can quantify real-time cellular oxygenation under hypoxic conditions and upon drug treatment. It is a great assay to investigate the interplay between cellular oxygenation & metabolism in research including hypoxia, cancer metabolism, and oxidative stress.


TMRE, MitoTracker and JC1 Mitochondrial Membrane Potential Assays

The TMRE , MitoTracker, and JC1 assays allows monitoring of the mitochondrial membrane potential in whole cells, which is also a good indicator of mitochondrial function.


MitoSOX assay

Reactive oxygen species (ROS) production is increased with oxidative stress, resulting in oxidative damage. With the MitoSOX assay the superoxide concentration inside the mitochondria can be assessed, providing an indication of ROS toxicity.


CellROX assay

The CellROX assay is a novel fluorogenic probe for measuring oxidative stress in live cells. Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to scavenge them.

Case Study

Ischemia reperfusion injury

During myocardial infarction the supply of oxygen in the parts of the heart is decreased or stopped by reduced or arrested blood flow. The condition of reduced oxygen concentration in a tissue is called ischemia. During the ischemic period the heart has to adopt its metabolism and is forced to generate ATP through anaerobic processes, which is mainly cytosolic glycolysis. This adoption prevents the heart from major damage. Most of the damage/cell death caused by the myocardial infarction develops during the so-called reperfusion when the blood flow is suddenly re-established and oxygen is again supplied to the former ischemic tissue.

Ischemia reperfusion like processes can be modeled in cardiomyocytes incubated in an atmospheric control unit (ACU) that can rapidly reduce the oxygen levels (infarction phase), maintain oxygen at low levels (ischemic phase) and rapidly increase oxygen levels (reperfusion phase).

Real time consumption of intracellular oxygen in iPSC-derived cells with different metabolic states

 

An example of how real time consumption of intracellular oxygen occurs in cells with different metabolic states: Cell oxygenation traces from cardiomyocytes that are respiring and consume intracellular oxygen (light red) and from cardiomyocytes that are not respiring (dark red). The black trace represents the oxygen level inside the plate reader chamber.

Assay Capabilities

Spark Multimode microplate reader system Novocyte flow cytometer systemImageXpress micro confocal system

Interested?

Would you like to know more about our Metabolism assay service? Or are you in search of another metabolic assay service than the ones listed above?

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Related

Research program 

to develop and launch a cell metabolism analysis platform

Case study 

Phenotypic screen of hypertrophic cardiomyopathy