---
title: "Application note: Ncyte® Heart in a Box"
description: This protocol describes the assembly and culturing of Ncyte Heart in a Box, human iPSC-derived 3D cardiac microtissues amenable for highthrougput screening
---

[Resources ](https://www.ncardia.com/insights/resources)

# [Application note: Ncyte® Heart in a Box](https://www.ncardia.com/insights/resources/application-note-heart-in-a-box)

 Written by [Ncardia Stem Cell Experts](https://www.ncardia.com/insights/resources/author/ncardia) | February 17, 2025

The [Ncyte® Heart in a Box™](https://www.ncardia.com/cell-supply/ncyte-heart-in-a-box) represents a groundbreaking advancement in cardiac research and drug discovery by providing a highly relevant, human-derived 3D model of the heart.

Unlike traditional 2D cell culture models, which often fail to replicate the complexity of human tissues, the Ncyte® system integrates three essential cell types—ventricular cardiomyocytes, endothelial cells, and cardiac fibroblasts—into a cohesive, functional model that mimics key aspects of human heart physiology and pathology. 

[View full post](https://www.ncardia.com/insights/resources/application-note-heart-in-a-box)

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