CNS.4U® Kit

CNS.4U® represent a highly physiological in vitro co-culture model of neurons and astrocytes for a range of drug development, neurotoxicity and disease modeling applications. The Kit contains 1 vial of cryopreserved, human iPSC-derived CNS.4U® neurons and astrocytes (≥ 2 million cells), Neuro.4U Basal Medium A (250 mL) and 2 vials Neuro-Supplement 2 (1.25 mL).


Product Information

CNS.4U® comprise of two key cell types of the human central nervous system (CNS): neurons (glutamatergic, GABAergic and dopaminergic) and astrocytes. The cells are simultaneously generated from neural precursor cells and recapitulate the interplay between excitatory and inhibitory neurons. Their rapid formation of neuronal networks, prolonged viability in culture, and availability in unlimited volumes, makes CNS.4U® advantageous compared to currently used primary cells and animal models for neuroscience-related applications. CNS.4U® represent an ideal tool for early and late drug discovery for neurodegenerative disease and epilepsy, in vitro neurotoxicity assays, and disease modeling, through electrophysiological, metabolic and imaging-based assays. We are a member of the FDA - HESI Neurotox for seizure liability and CNS.4U® will be validated through this initiative.

Product Data

Product Specifications

CNS.4U® is a heterogeneous group of neuronal and glial cells (~30-40% glutamatergic, ~30-40% GABAergic, ~10% dopaminergic and ~10% astrocytes depending on the culture conditions). CNS.4U® is able to recapitulate the interplay of excitatory and inhibitory neurons in vitro. In MEA recordings, CNS.4U® neurons show robust spontaneous and burst-like neuronal activity after 10 days in cultivation. We consistently observe calcium oscillations (FDSS µCell) and network burst activity (MEA) in CNS.4U® cultures at week 4 – 4.5. Neurons with supporting astrocytes within the same culture provide an easy-to-use human iPSC-derived neuronal model for seizure liability assays standardized by Ncardia participation in the FDA-HESI Neurotox initiative.


Catalog number

Ax-B-HZ02-2M kit

Production Technology

Scalable; large lot sizes with minimal batch to batch variability guaranteed by an extensive quality control of identity (immunostaining) and functionality (MEA analysis)


Heterogeneous cultures


Post-mitotic neural phenotype

Assay window

Neuronal spike activity (MEA) after day 10; Calcium oscillations (FDSS µCell) and network burst activity (MEA) at week 4


Robust spontaneous and burst-like neuronal activity of CNS.4U® neurons present in MEA recordings. Neuronal network formation indicated by calcium oscillations and network burst activity established in cultivation week 4.

Seizure Liability Analysis

Dose dependent responsiveness to seizure-related compounds, i.e. positive control increases mean firing rate of the neurons, number of network bursts and amplitude of network burst while negative control reduces mean activity, network burst and amplitude of network burst.

Recommended Storage

CNS.4U® Cells: liquid nitrogen

Neuro.4U® Basal Medium A: 4°C

CNS.4U® Supplement: -80°C