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Ncyte Cardiomyocytes

Human iPSC-derived ventricular-like cardiomyocytes


Immunofluorescence staining of Ncyte cardiomyocytes. Cardiac Troponin T (Green) and DAPI (Blue).
Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes are an in vitro platform that can provide a better solution for mechanistic analysis, and drug safety and efficacy screenings. Ncyte Cardiomyocytes are fully functional human iPSC-derived ventricular-like cardiomyocytes, obtained using Ncardia’s controlled bioreactor technology. Their relatively slow beat rate enables pacing for electrophysiological research. Ncyte Cardiomyocytes are a clinically relevant in vitro model compatible with an array of functional assays, such us calcium-imaging, contractility force evaluation and metabolism studies.

 

Cellular Identity

 

Ncyte Cardiomyocytes are characterized by flow cytometry to ensure a purity of ≥ 70% cardiac TroponinT (cTnT) positive cells after 3 days in culture following Ncardia’s user guide (See figure A). They typically express Myosin Light Chain 2v (MLC2v), indicating a ventricularlike phenotype (See figure B). Based on RNA-seq data, Ncyte Cardiomyocytes express common sarcomere markers, such as TNNT2, while hiPSC and cardiac progenitor markers are silenced. Expression levels for additional genes are available upon request.


A) Flow cytometry analysis of one representative batch showing cTnT positive cells (99%) and cTnT/MLC2v double positive cells (76%). B) Histograms of cTNT and MLC2v fluorescence signal and their antibody controls.

Physiologically Relevant

 

Ncyte Cardiomyocytes exhibit well aligned myofibrils and intact structural sarcomere organization (See figure A). Their action potential shows fast upstroke velocity and a well-defined plateau phase, resembling human ventricular cardiomyocytes (See figure B). These make the Ncyte Cardiomyocytes a physiologically relevant model for the phenotypic study of cardiac diseases. Our expert scientists can develop and execute predictive assays to support your drug discovery research in the cardiac field.


Immunofluorescence staining of thin and thick myofilament proteins of Ncyte Cardiomyocytes: Alpha-actinin (Red), myosin heavy-chain 7 (Green) and DAPI (Blue).

Representative patch clamp action potential for one batch of Ncyte Cardiomyocytes in current clamp mode.

Highly suitable for electrophysiological research

 

Ncyte Cardiomyocytes present a relatively slow and uniform beating rate, which enables pacing for electrophysiological research (See top figure). Additionally, response to adrenergic stimulation with isoproterenol and ion channel blockers, nifedipine (L-type calcium channel blocker) and dofetilide (hERG channel blocker), is guaranteed for every batch (See figure A, B, C). Our scientific team is experienced in the electrophysiological analysis of Ncyte Cardiomyocytes for safety and efficacy studies and can report the analyzed results to you.


Microelectrode array (MEA) baseline beating rate of a 96-well plate with Ncyte Cardiomyocytes

A) 30-minute exposure to isoproterenol induced an increase in beat rate of Ncyte Cardiomyocytes. B) 30-min exposure to Dofetilide, a hERG (IKr) channel blocker, induced a dose dependent prolongation of field potential duration as well as notable arrhythmias in Ncyte cardiomyocytes, at concentrations of ≥10 nM. C) 30-minute exposure to Nifedipine, an L-type calcium channel blocker, shortened the field potential duration of Ncyte cardiomyocytes in a dosedependent manner. Data presented as mean ±SD (n=6) of the % change to baseline recordings for each well and time-matched 0.1% DMSO control.

Applications for Drug Discovery

 

High-throughput screening with a human iPSC-derived model of cardiac hypertrophy

Read the case study