Ncardia Belgium
47 Rue Adrienne Bolland
6041 Gosselies
Belgium

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Human iPSC-derived Ncyte Cortical Neurons are post mitotic, glutamatergic, cortical layer-committed neurons. They express cortical progenitor marker FOXG1 and cortical layer markers TBR1 and Ctip2 (cortical layers V-VI), together with neuronal marker βIII Tubulin and dendrite marker MAP2. The kit includes cryopreserved human iPSC-derived cortical neurons, optimized medium and protocol.
Catalog number |
Nc-K-CN |
Kit contents |
≥ 4M vial Ncyte Cortical Neurons per vial
250 mL Ncyte Neuronal Basal Medium (Cat #: Nc-M-NBM)
2 vials of 1.25 mL Ncyte Neuronal Supplement (Cat #: Nc-MS-NNS) |
Key biomarkers |
FOXG1, TBR1, Ctip2, ßIII tubulin , MAP2 |
Assay window |
Electrical activity as early as day 4, as demonstrated by neuronal spike activity in MEA assays |
Storage recommendations |
Ncyte Cortical Neuron cells: liquid nitrogen Ncyte Neuronal Basal Medium: 2-8°C Ncyte Neuronal Supplement: -80°C |
Cortical neuron morphology at day 9 after seeding. Cells were seeded at 150,000 cells per cm2 on a Poly-L-ornithine/Laminin coated plate. Magnification 200x.
Ncyte Cortical Neurons express neuronal marker βIII Tubulin (red) and cortical neuron marker TRB1 (green) 7 days after thawing. Merged image with DAPI (nuclear marker).
Ncyte Cortical Neurons express neuronal marker βIII Tubulin (red) and cortical neuron marker Ctip2 (green) 7 days after thawing. Merged image with DAPI (nuclear marker).
Ncyte Cortical Neurons express neuronal marker βIII Tubulin (red) and mature neuron marker MAP2 (green) 7 days after thawing. Merged with DAPI (nuclear marker).