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Pluricyte
Cardiomyocyte Kit

Our Pluricyte Cardiomyocytes are functional human iPSC-derived cardiomyocytes without genetic modification, manufactured without purification / selection. They are cultured a serum-free, well-defined culture and maturation medium. The cells show highly-organized, aligned sarcomere structures and a relatively low beat rate.

  • High reproducibility enabled by strict quality control

  • Physiologically relevant model

  • Used in detecting proarrhythmic effects of compounds

Product Description

Catalog number

PCK-1.5

Kit contents

2M viable, cryopreserved Pluricyte Cardiomyocyte cells

100 mL Pluricyte Cardiomyocyte Medium (Cat. #: PM-2100-100mL)

Purity

>70% based on cardiac Troponin T expression. High purity of ventricular cardiomyocytes based on high MLC2v expression and manual patch clamp

Morphology

High degree of structural sarcomere organization

Assay window

1st contractions appear 24-48 hours post-thaw

Electrically coupled monolayer forms in 3-4 days post thaw

Stable beating monolayers observed 7-8 days post thaw (15-40 BPM)

Electrophysiology-based assays optimal between 8-12 days after plating

Single cell action potential

Low resting membrane potential (~-78 mV)

Fast upstroke velocity and robust action potential amplitude

Monolayer field potential

Well-pronounced and robust de- and repolarization peaks,
enabling easy detection of field potential durations and
facilitating MEA assay analysis for pro-arrhythmia drug liability assessment studies.

Recommended Storage

Cryopreserved cells: liquid nitrogen vapor phase

Pluricyte Cardiomyocyte Medium: -20°C, after opening 4°C

Product Data

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