x

Calcium Transients

Our Calcium Transients Service provides cardiac safety assessment by monitoring spontaneous Ca2+ transients in human iPSC-derived cardiomyocytes with the capability for high throughput (HT). Acute and long-term drug effects on the electrophysiology of cardiomyocytes can be detected. The service expedites decision making at early stages of preclinical drug development.

 

What are your benefits?

High throughput screening
Enabling screening in early drug development

Fully functional model
Proven effects of mechanistically diverse reference compounds

Reproducible assay
Enabled by fully automated assay execution

Introduction

The Calcium Transients are based on fluorescence-based assays that measure changes of intracellular calcium transient parameters. Since calcium is the interdependent regulator between cardiomyocyte electrophysiology and contraction, compounds affecting ion channels can also affect intracellular calcium transients.

For this assay, the human iPSC-derived cardioymocytes are cultured in standard 96 or 384 well plates and loaded with a calcium sensitive dye. All cardiomyocyte culturing steps, including plate coating, cell seeding and medium refreshment will be performed in a fully automated manner, ensuring high assay reproducibility. Compound effects are assessed on the FDSS/µCell Instrument (Hamamatsu).

Workflow

Interested?

Would you like to know more about our Calcium Transients Service, or any of our other services?

We're here to listen to your project plans, questions and challenges, and to co-develop a solution that fits your specifications. Please leave your details and message in the form below and we will get back to you as soon as possible.