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Endothelial Barrier Toxicity

Gain detailed understanding of compound effects on vascular barrier function through our Endothelial Barrier Toxicity Service. This highly relevant, sensitive and predictive in vitro safety assessment of drug candidates is performed using human iPSC-derived endothelial cells. The assay includes high-content, real-time and label-free impedance-based technology (ECIS) by monitoring changes in electrical impedance across the endothelial cell monolayer.

 

What are your benefits?

Evaluation of concentration- and time-dependent compound effects
Enabled by non-invasive real-time data acquisition

Reproducible
Assay based on quality-controlled hiPSC-derived endothelial cells with low batch-to-batch variation

Translatable
hiPSC-derived endothelial cells are a physiologically relevant model

Introduction

Vascular endothelial cells form the inner lining of blood vessels and are key for normal physiological functioning of tissues. In healthy conditions, these cells form a delicate barrier for tight regulation of the exchange of nutrients and metabolic waste between the blood circulation and surrounding tissues. Dysregulation of this barrier function leads to edema and ultimately to organ failure, which is considered a major dose-limiting adverse effect of many therapeutics under development.

Our advanced data analysis capabilities enable calculation of time course changes in the barrier function of the cell layer, the degree of constricted flow of current under the cells and the cell membrane capacitance. Such analyses enable discrimination between necrosis and apoptosis, between subtle loss of cell-cell contacts or acute cell death, or the detection of a subtle loss of cell-cell contacts which cannot be assessed when using overall resistance.

Workflow

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