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Contractility Safety Analysis

Our contractility safety analysis service offers a non-invasive in vitro assay to measure true contractility of human cardiomyocytes with exceptional accuracy under physiological mechanical boundary conditions. In a customized assay format also co-cultures of the cardiomyocytes with hiPSC-derived cardiac fibroblasts can be applied.

The system provides an ideal tool to assess pharmacological, safety pharmacological and toxicological effects of drug candidates on human cardiomyocytes.

What are your benefits?

The only true force measurement
on the market at medium-throughput

Sensitive and predictive
for cardiac safety pharmacology

Introduction

hiPSC-derived cardiomyocytes are cultured on plates featuring a flexible culture substrate composed of a ultra-thin silicone membrane. Together with these culture plates the custom made assay system allows for measurements and quantification of the contraction-induced deflection of the silicone membranes. This is facilitated by a combination of capacitive distance sensors and a pressure sensors beneath the membrane of each well. All measurements are performed in a cell culture incubator at 37°C and 5% CO2.


Workflow

Service specifications

Cell type

iPSC-derived cardiomyocytes

Species

Human

Service type

Biomechanical analysis

Format

8 wells (on 24 well plate)

Time points

5-15 minutes after compound incubation

Compound concentrations*

0.001 µM – 10 µM (in 5 logarithmic steps) in medium + 0.1 % DMSO

 

Positive control

Isoprenaline 0.001 µM – 0.1 µM; Verapamil 0.1 µM – 1 µM (in duplicates)

Vehicle control

0.1 % DMSO (in duplicates)

Parameters

Highest dose - (optional washout)

Timelines

7 weeks for up to 5 compounds (in quadruplicates)

Deliverable

A study protocol will be agreed before study initiation. Results will be presented in a final study report

* Suggested concentrations, to be agreed with client

Interested?

Would you like to know more about our Contractility Analysis Service, or any of our other services?

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