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Endothelial Barrier Toxicity

Ncardia’s Endothelial Barrier Toxicity Assay offers a highly relevant, sensitive and predictive in vitro safety assessment of drug candidates using functional human endothelial cells, derived from induced pluripotent stem cells. The assay includes high-content, real-time and label-free impedance-based technology (ECISTM) that allows detailed understanding of compound effects on vascular barrier function (which is deteriorated in drug-induced vascular leakage) by monitoring changes in electrical impedance across the endothelial cell monolayer.

 

What are your benefits?

Evaluation of concentration- and time-dependent compound effects
Enabled by non-invasive real-time data acquisition

Reproducible
Assay based on quality-controlled hiPSC-derived endothelial cells with low batch-to-batch variation

Translatable
hiPSC-derived endothelial cells are a physiologically relevant model

Introduction

Our advanced data analysis capabilities enable calculation of time course changes in the barrier function of the cell layer (Rb; permeability), the degree of constricted flow of current under the cells (Alpha; cell-matrix interaction) and the cell membrane capacitance (Cap; a measure of cell viability). Such analyses enable discrimination between necrosis and apoptosis, between subtle loss of cell-cell contacts or acute cell death, or the detection of a subtle loss of cell-cell contacts which cannot be assessed when using overall resistance.

Workflow

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