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Structural Cardiotoxicity - Troponin Detection

Ensure a better prediction of your compounds' induced cardiotoxic risks, based on biomarker detection. Our Troponin Detection Service offers an early structural cardiotoxicity assay based on fully validated and functional human iPSC-derived cardiomyocytes and is suitable for high-throughput safety and toxicity screening of your test compounds.

What are your benefits?

Clinically relevant Troponin I biomarker
Currently most widely used biomarker for detection of myocardial damage in patients

High sensitivity
Detection as low as 10 pg/mL

Kinetic compound analysis
Enabled by the non-destructive nature of the assay

Enabling multiplexed assays
Compatible with a wide range of electrophysiology assays such as MEA, impedance or Ca2+-flux

Introduction

Drug-induced cardiotoxicity can be functional or structural in nature, and should both be investigated to ensure a better prediction of induced cardiotoxic risks. Cardiac troponin is a sensitive and specific biomarker for assessing structural cardiotoxicity. We determine cardiac troponin by detection of the secreted cardiac specific marker Troponin I, which is released into circulation after cardiomyocyte damage induced by myocardial infarct or after treatment with cardiotoxic drugs. Instead of secreted Troponin I, other biomarkers can also be detected upon request.

For a more comprehensive assessment of predicting cardiotoxicity, this assay can be multiplexed with any functional readout including microelectrode array (MEA), impedance or calcium transient assays.

Workflow

Service specifications

Cell type

Human iPSC-derived cardiomyocytes

Service type

Structural toxicity based on biomarker detection

Format

96 wells (Up to 384 wells possible)

Time points

16, 24, 40, 48 and 64 hours after compound addition

Compound concentration*

0.1, 1, 10 µM in medium + 0.1% DMSO

Positive control

Ponatinib

Vehicle control

0.1% DMSO

Parameters

Released Troponin I detection

Timelines

3 Weeks for up to 9 compounds (in triplicates)

Deliverable

A study protocol will be agreed before study initiation. Results will be presented in a study report.

* Suggested concentrations, to be agreed with client

Interested?

Would you like to know more about our Structural Cardiotoxity Detection Service, or any of our other services?

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