This poster presents Ncardia’s development and characterisation of human iPSC-derived 3D neurospheres, combining neurons, astrocytes and microglia to investigate neuronal maturation, network activity and disease-related pathology. The study compares 3D neurospheres with 2D neuronal cultures and explores their application in neurodegenerative disease modelling.
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How human iPSC-derived neurons, astrocytes and microglia are combined to generate 3D neurospheres, with characterisation of cellular composition and synaptic development
How multi-electrode array (MEA) recordings reveal differences in neuronal firing patterns between 2D and 3D cultures, including complex bursting and synchronised network activity
How synaptic markers, including synaptophysin (SYP) and PSD-95, develop over time in 3D neurospheres, providing insights into neuronal maturation
How 3D neurospheres can be adapted for neurodegenerative disease modelling, demonstrated through increased Tau and phosphorylated Tau staining following transduction