Advanced Research Series
Next-Generation iPSC Models Stem From Ncardia
Identity
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High-purity iPSC-derived hematopoietic stem/progenitor cells (≥70% CD34+/CD45+/CD43+)
- Stepwise differentiation confirmed by surface marker expression and flow cytometry
- Suitable for applications in hematopoiesis research, immunology, disease modeling and drug testing
| Identity Markers | ≥70% CD34+/CD45+/CD43+ |
| Size | ≥ Variable formats |
| Quality Control | Cell count, Viability, Identity (FACS), Mycoplasma |
| Format | Cryopreserved cells |
| Donor | Female |
| Reprogramming method | Non-viral |
| Shipping conditions | Dry shipper, −180°C to −135°C |
| Storage conditions | Vapor phase of liquid nitrogen |
Cellular identity
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Our HSCs show efficient differentiation, with >97% CD34+ cells at early stages
- Flow cytometry confirms sequential acquisition of CD43 and CD45, resulting in >90% CD34+/CD43+ and >90% CD34+/CD45+ definitive hematopoietic populations
- This progressive enrichment is maintained across batches and is stable post-thaw, ensuring consistency and robustness for downstream applications
Product Information and Applications
User Guide
This protocol covers thawing, seeding, and culturing of iPSC-Hematopoietic Stem Cells.
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Identity
- High-purity lung progenitor cells (≥70% CPM+/NKX2.1+) generated in a controlled bioreactor system
- Display physiologically relevant gene expression and functionality for developmental and disease modeling studies
- Highly suitable for applications in respiratory drug discovery, toxicity testing and regenerative medicine research
| Identity Markers | ≥70% CPM+/NKX2.1+ |
| Size | Variable formats |
| Quality Control | Cell count, Viability, Identity (FACS), Mycoplasma, Expansion |
| Format | Cryopreserved cells |
| Donor | Female |
| Reprogramming method | Non-viral |
| Shipping conditions | Dry shipper, −180°C to −135°C |
| Storage conditions | Vapor phase of liquid nitrogen |
Cellular identity
- Lung progenitor cells derived from iPSCs show robust expression of NKX2.1, a key transcription factor for lung lineage; flow cytometry demonstrates high proportions of NKX2.1+ cells (>85%), validating their progenitor identity
- Gene expression analysis confirms strong upregulation of NKX2.1 during directed differentiation from iPSCs through DE and AFE stages to LPCs; this stepwise process ensures reproducibility and lineage fidelity
- Ready-to-use for respiratory biology and drug discovery
Product Information and Applications
Need More Technical Information?
Our technical team can provide the complete user guide and answer any questions about culturing and handling Lung Progenitor Cells.
Speak with an ExpertIdentity
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High-purity ventricular-like cardiomyocytes produced in a controlled bioreactor system
- Physiologically relevant in electrophysiology, calcium, and metabolic assays
- Highly suitable for drug screening
| Identity Markers | ≥90% TNNT2 at day 3 of culture according to user guide |
| Size | ≥8M viable cells at day 3 of culture according to user guide |
| Quality Control | Cell count, Viability, Identity (FACS), Functionality (MEA: Dofetilide, Nifedipine, Isoproterenol), Mycoplasma |
| Format | Cryopreserved cells |
| Donor | Female |
| Reprogramming method | Non-viral |
| Shipping conditions | Dry shipper, −180°C to −135°C |
| Storage conditions | Vapor phase of liquid nitrogen |
Want to know more about Ncyte® vCardiomyocytes?
Learn more about consistency, gene expression, functional assessments and other product details.
Take a Closer LookProduct Information and Applications
User Guide
This protocol covers thawing, seeding, and culturing of iPSC-vCardiomyocytes.
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Identity
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High-purity iPSC-derived alveolar type II epithelial cells (≥70% Pro-SPC+/ABCA3+) produced in a scalable bioreactor system
- Express key AT2 markers (Pro-SPC, ABCA3) and retain proliferative capacity in vitro
- Functionally relevant for pulmonary disease modeling, fibrosis, drug testing and toxicity studies
| Identity Markers | ≥70% Pro-SPC+/ABCA3+ |
| Size | Variable formats |
| Quality Control | Cell count, Viability, Identity (FACS), Mycoplasma |
| Format | Cryopreserved cells |
| Donor | Female |
| Reprogramming method | Non-viral |
| Shipping conditions | Dry shipper, −180°C to −135°C |
| Storage conditions | Vapor phase of liquid nitrogen |
Specifications
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Our AT2 cells show robust expansion potential, with proliferation increasing steadily over a 7-day culture period.
- Flow cytometry analysis confirms high expression of Pro-SPC in the majority of cells, supporting their AT2 lineage identity.
- Strong ABCA3 expression further validates the presence of the lamellar body-associated phenotype characteristic of functional AT2 cells.
- Functional activity is demonstrated by secretion of surfactant protein C (SPC), as detected by ELISA in culture supernatants, confirming their physiological relevance.
Product Information and Applications
User Guide
This protocol covers thawing, seeding, and culturing of iPSC-Alveolar Type II Cells.
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Identity
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High-purity cardiac fibroblasts-like cells produced in a scalable bioreactor system
- Express key fibroblast and cardiac stomal markers and display typical spindle - shaped morphology
- Functionally relevant for cardiac tissue modeling, fibrosis, drug testing, and co-culture systems
| Identity Markers | ≥70% VIM, expression of CX43 and TCF21 |
| Size | ≥1M cells/vial at day 3 post-thaw |
| Quality Control | Cell count, Viability, Identity (FACS), Mycoplasma |
| Format | Cryopreserved cells |
| Donor | Male |
| Reprogramming method | Non-viral |
| Shipping conditions | Dry shipper, −180°C to −135°C |
| Storage conditions | Vapor phase of liquid nitrogen |
Specifications
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Across production runs, NHP iPSC–cardiac fibroblasts consistently achieve ≥70% VIM+ cells, confirming stable fibroblast identity
- Flow cytometry analysis demonstrates robust expression of key cardiac fibroblast markers, including VIM (~84%) and TCF21 (~71%), supporting reproducible differentiation outcomes.
- Immunofluorescence imaging confirms co-expression of VIM and CX43, alongside typical fibroblast morphology, indicating functional relevance for cardiac stromal applications.
Product Information and Applications
User Guide
This protocol covers thawing, seeding, and culturing of NHP-C Cardiac Fibroblasts.
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