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Ncyte® Heart in A Box

Human iPSC-derived 3D cardiac microtissue

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Ncyte® Heart in a Box™ is an innovative 3D cardiac microtissue model derived from human-induced pluripotent stem cells (iPSCs). It integrates three critical cell types—ventricular cardiomyocytes, endothelial cells, and cardiac fibroblasts—into a single, high-purity product that mirrors the complexity of the human heart. 

This system provides a more accurate platform for studying heart development, disease modeling, and therapeutic testing.

This model is based on Giacomelli E,  et al. Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease. Cell Stem Cell. 2020 Jun 4;26(6):862-879.e11. doi: 10.1016/j.stem.2020.05.004.

Benefits
  • Comprehensive cardiac model
  • High purity and functionality
  • Supports co-culture applications to study cell interactions within the cardiac microenvironment
  • Enables advanced toxicity and drug screening studies
cardiac
Immunofluorescence staining for α-SMA (red), cTnT (yellow), and PECAM (green) showing the presence of key cell types. DAPI (blue) stains nuclei.
 

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Product Specifications

Identity markers

vCardiomyocytes: ≥90%cTnT+, Endothelial Cells: ≥90% CD31+/CD144+, Cardiac Fibroblasts: ≥95% vimentin+/≥85% TCF21, Thawing according to user guide

Format

Cryopreserved cells

Size (viable cells/vial)

vCardiomyocytes: ≥4M viable cells, Endothelial Cells: ≥1M viable cells, Cardiac Fibroblasts: ≥ 0.4M cells viable cells, Thawing cells according user guide

Quality Control

Cell count, Viability, Identity (FACS, ICC), Mycoplasma testing

Donor

Female

Reprogramming method

Ectopic expression of reprogramming factors using episomal plasmids

Shipping conditions

Dry shipper, -180C to -135C

Storage conditions

Vapor phase of liquid nitrogen

Technical Data

Learn more about Ncyte® Heart in a Box by downloading the fact sheet

Cardiotoxicity Testing and Drug Screening

Ncyte® Heart in a Box™ offers a comprehensive platform for assessing the cardiotoxic effects of potential drug candidates. By incorporating cardiomyocytes, endothelial cells, and cardiac fibroblasts, this system allows researchers to evaluate drug effects on cardiac function, viability, and toxicity across various heart cell types. High-throughput screening and large-scale compound testing can predict cardiotoxicity risks before clinical trials, reducing the reliance on animal models.

Heart Disease Modeling

This system is ideal for modelling various
cardiovascular diseases, including ischemic
heart disease, heart failure, hypertrophy, and
fibrosis. Researchers can explore disease
mechanisms in a human-relevant 3D envi-
ronment, gaining a deeper understanding of
cellular interactions and disease progression.
Ncyte® Heart in a Box™ is particularly useful
for studying how cardiomyocytes, endothelial
cells, and fibroblasts interact during heart
disease development.

Response to Drugs in the Heart in a Box Model

Angiogenesis and Vascular Remodeling

Ncyte® Endothelial Cells form functional
vascular networks, providing an excellent
model for studying angiogenesis and vascular
remodelling, especially in ischemic heart
diseases. This application is valuable for
testing therapies aimed at promoting blood
vessel growth and improving circulation,
such as in post-myocardial infarction or
heart failure.

Ac-LDL uptake assay showing Ncyte® Endothelial Cells’ ability to internalize acetylated low-density lipoprotein

Fibrosis Research and ECM Remodeling

Cardiac fibrosis, a hallmark of many heart
diseases, is driven by the activation of car-
diac fibroblasts. Ncyte® Cardiac Fibroblasts
offer a unique opportunity to study ECM re-
modelling, collagen deposition, and fibroblast
activation in response to injury. This system
is critical for developing antifibrotic therapies
to reduce scar tissue formation and restore
heart function.