Ncyte® Microglia
Ncyte hiPSC-derived Microglia are high-purity microglial cells that demonstrate phagocytosis and cytokine release, closely mimicking the behavior of human microglia. They offer researchers a reliable, accessible, and physiologically relevant platform to study microglial interactions, responses to central nervous system injuries, and disease mechanisms.
Especially suited for co-culture and neuroinflammation studies, they support the advancement of therapeutic research and development for CNS disorders and a range of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and ALS.

Accelerate drug discovery by rapidly evaluating therapeutic compounds targeting neuroinflammation
- Rapidly evaluate therapeutic compounds
- Effectively mediate inflammatory responses
- Valuable for investigating cytokine release in neuroinflammatory conditions.
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Product Specifications
Identity markers
≥80% TREM2+/CD11b+, ≥90% CD45+/CD46+, ≥70% IBA1 at thawing, according to user guide
Size
≥ 1.5 M viable cells at thawing, according to user guide
Quality Control
Cell count, Viability, Identity (FACS, ICC), Cytokine release, Mycoplasma
Format
Cryopreserved cells
Donor
Female
Reprogramming method
Non-viral
Shipping conditions
Dry shipper, -180°C to -135°C
Storage conditions
Vapor phase of liquid nitrogen
Technical data
- Immunofluorescence staining demonstrates robust expression of microglial markers including TREM2, IBA1, PU.1, TMEM119, and CX3CR1 in Ncyte® Microglia.
- Confocal microscopy reveals co-localization of microglial markers, indicating the presence of a mature microglial phenotype.
- Ncyte® Microglia exhibit characteristic ramified morphology, further supporting their microglial identity.



- Morphological evidence of activation is demonstrated by changes in Ncyte® Microglia morphology following LPS stimulation.
- Flow cytometry and ICC confirm robust expression of key microglial markers (CX3CR1, TREM2, IBA1, CD45, CD11b) in both resting and activated states.
- Ncyte® Microglia show significant cytokine release upon LPS stimulation, highlighting their functional role in mediating inflammatory responses.
- Ncyte® Microglia offer an ideal in vitro model for studying neuroinflammation and the mechanisms of neurodegenerative diseases, including
Alzheimer’s and Parkinson’s.

- Ncyte® Microglia exhibit a strong pro-inflammatory reaction upon LPS stimulation.
- LPS treatment significantly increases the production of IL-6 and TNF-α, key inflammatory mediators. These results underscore the ability of Ncyte® Microglia to effectively mediate inflammatory responses.
- Ncyte® Microglia provide a valuable in vitro model for investigating cytokine release in neuroinflammatory conditions.

Certificates of analysis are available upon request via support@ncardia.com
Our work centers on a simple yet powerful premise:
When we combine deep iPSC knowledge, broad assay capabilities and a demonstrated ability to integrate the biology of human diseases into preclinical research, we can help drug developers make critical decisions earlier and with more confidence.

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