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Ncyte® Microglia

Human iPSC-derived Microglia

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Ncyte hiPSC-derived Microglia are high-purity microglial cells that demonstrate phagocytosis and cytokine release, closely mimicking the behavior of human microglia. They offer researchers a reliable, accessible, and physiologically relevant platform to study microglial interactions, responses to central nervous system injuries, and disease mechanisms.

Especially suited for co-culture and neuroinflammation studies, they support the advancement of therapeutic research and development for CNS disorders and a range of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and ALS.

Benefits
  • Rapidly evaluate therapeutic compounds
  • Effectively mediate inflammatory responses
  • Valuable for investigating cytokine release in neuroinflammatory conditions
neural
Accelerate drug discovery by rapidly evaluating therapeutic compounds targeting neuroinflammation.
 

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Product Specifications

Identity markers

≥80% TREM2+/CD11b+, ≥90% CD45+/CD46+, ≥70% IBA1 at thawing, according to user guide

Size

≥ 1.5 M viable cells at thawing, according to user guide

Quality Control

Cell count, Viability, Identity (FACS, ICC), Cytokine release, Mycoplasma

Format

Cryopreserved cells

Donor

Female

Reprogramming method

Non-viral

Shipping conditions

Dry shipper, -180°C to -135°C

Storage conditions

Vapor phase of liquid nitrogen

Technical data

Identity Ideal model for neuroinflammation Robust cytokine release
  • Immunofluorescence staining demonstrates robust expression of microglial markers including TREM2, IBA1, PU.1, TMEM119, and CX3CR1 in Ncyte® Microglia.

  • Confocal microscopy reveals co-localization of microglial markers, indicating the presence of a mature microglial phenotype.

  • Ncyte® Microglia exhibit characteristic ramified morphology, further supporting their microglial identity.
Expression of CX3CR1 and TREM2 suggests suitability for co-culture systems with neurons to model Alzheimer's Disease and other neurodegenerative diseases.
Brightfield images of Ncyte® Microglia on day 7 post-thaw. A) Untreated microglia and B) Microglia treated with LPS (100 ng/ml) for 18hrs.
  • Morphological evidence of activation is demonstrated by changes in Ncyte® Microglia morphology following LPS stimulation.

  • Flow cytometry and ICC confirm robust expression of key microglial markers (CX3CR1, TREM2, IBA1, CD45, CD11b) in both resting and activated states.

  • Ncyte® Microglia show significant cytokine release upon LPS stimulation, highlighting their functional role in mediating inflammatory responses.

  • Ncyte® Microglia offer an ideal in vitro model for studying neuroinflammation and the mechanisms of neurodegenerative diseases, including
    Alzheimer’s and Parkinson’s.

 

  • Ncyte® Microglia exhibit a strong pro-inflammatory reaction upon LPS stimulation.

  • LPS treatment significantly increases the production of IL-6 and TNF-α, key inflammatory mediators. These results underscore the ability of Ncyte® Microglia to effectively mediate inflammatory responses.

  • Ncyte® Microglia provide a valuable in vitro model for investigating cytokine release in neuroinflammatory conditions.
Product Information and Applications

 

Certificates of analysis are available upon request via support@ncardia.com

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Our work centers on a simple yet powerful premise: 
When we combine deep iPSC knowledge, broad assay capabilities and a demonstrated ability to integrate the biology of human diseases into preclinical research, we can help drug developers make critical decisions earlier and with more confidence.