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Ncyte® vCardiomyocytes

Human iPSC-derived ventricular-like cardiomyocytes

Ventricular cardiomyocytes generated with our standardized process are fully functional. Their relatively slow beat rate enables pacing for electrophysiological research. Base on this model, an array of functional assays can be developed and optimized providing relevant predictions of drug-induced responses.

Our team can generate cardiomyocytes from the iPSC that best fits your project or work with off-the-shelf Ncyte® vCardiomyocytes to support your projects.

cardiac
Immunofluorescence staining of Ncyte® vCardiomyocytes. Cardiac Troponin T (Green), alpha-actinin (Red) and DAPI (Blue).
Benefits
  • High purity ventricular-like cardiomyocytes
  • Physiologically relevant
  • Highly suitable for electrophysiological research

Do you want to buy Ncyte® vCardiomyocytes?

Do you need a custom cell model?

Product Specifications

Identity markers

≥ 70% TNNT2 at day 3 of culture according user guide

Size

≥ 4M viable cells at day 3 of culture according to user guide

Quality Control

Cell count, Viability, Identity (FACS), Functionality (MEA), Mycoplasma

Format

Cryopreserved cells

Donor

Female

Reprogramming method

Non-viral

Shipping conditions

Dry shipper, -180°C to -135°C

Storage conditions

Vapor phase of liquid nitrogen

Supplied with

Ncyte® Cardiomyocyte Medium

Technical Data

Ncyte® vCardiomyocytes are characterized by flow cytometry to ensure a purity of ≥ 70% cardiac TroponinT (cTnT) positive cells after 3 days in culture following Ncardia’s user guide (Figure 2A). They typically express Myosin Light Chain 2v (MLC2v), indicating a ventricular-like phenotype (Figure 2B). Based on RNA-seq data, Ncyte® vCardiomyocytes express common sarcomere markers, such as TNNT2, while hiPSC and cardiac progenitor markers are silenced. Expression levels of additional genes are available upon request.

A) Flow cytometry analysis of one representative batch showing cTnT positive cells (99%) and cTnT/MLC2v double positive cells (76%). B) Histograms of cTNT and MLC2v fluorescence signal and their antibody controls.

Ncyte® vCardiomyocytes exhibit well aligned myofibrils and intact structural sarcomere organization (See figure A). They typically express Myosin Light Chain 2v (MLC2v), indicating a ventricularlike phenotype. Based on RNA-seq data, Ncyte® vCardiomyocytes express common sarcomere markers, such as TNNT2, while hiPSC and cardiac progenitor markers are silenced. Expression levels of additional genes are available upon request.

 

Their action potential shows fast upstroke velocity and a well-defined plateau phase, resembling human ventricular cardiomyocytes (See figure B,C).

 

Ncyte® vCardiomyocytes present a relatively slow and uniform beating rate, which enables pacing for electrophysiological research. 

Our scientific team is experienced in pharmacology and toxicity assays of cardiomyocytes to help you select the best drug candidates.

Response to adrenergic stimulation with isoproterenol and ion channel blockers, nifedipine (L-type calcium channel blocker) and dofetilide (hERG channel blocker), is guaranteed for every batch.

 

For more data download the fact sheet