How Does STAR-CRISPR Measure Up?
CRISPR-based systems have two key components:
- Guide RNA (gRNA) — a short RNA sequence matching the target DNA sequence to be edited
- DNA cutting enzyme — typically Cas9 or their derivatives
Among all the CRISPR-based systems available to edit iPSCs, STAR-CRISPR stands out with:
Unparalleled efficiencies in gene knock-in and knock-out
Minimal off-target editing
STAR-CRISPR offers about a 90% of Knock-out efficiency, surpassing Cas9 and MAD7
Knock-in efficiency is 2–3 times higher than other leading CRISPR-based methods when applied to iPSCs
STAR-CRISPR significantly mitigates the risk of unintended edits, also called off-targets, when compared to Cas9 in iPSCs
