"High-throughput in vitro assays to model neurodegeneration with human iPSC-derived neuronal and glial cells"
Isabel Onofre, PhD and Laura Calo, PhD
Despite the great potential of human induced pluripotent stem cells (hiPSCs), technical and conceptual challenges for disease-specific assay validation often hinder their use during pre-clinical development.
Ncardia has established large-scale manufacturing processes for hiPSC-derived neuronal sub-populations, microglia and astrocytes and has extensively characterized them using high-content imaging, multi-electrode arrays, flow-cytometry, and transcriptome analysis.
In this webinar, we show validated assays with co-culture of neurons, astrocytes and microglia in multiplexed and sensitive platforms where cell viability, autophagy, mitochondrial function, Ca2+ oscillations, endoplasmic reticulum stress and synaptic function can be perturbed to model neuro-pathological signatures.
These validated platforms provide ideal tools for the screening of novel therapeutic modalities against neurodegenerative pathologies.
In this webinar you will learn:
- The benefits and challenges of hiPSC-derived neuronal models
- How to describe the CNS pathophysiological changes associated with disease
- Optimized assays that recapitulate disease-specific phenotypes
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