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Ncyte® vCardiomyocytes

Human iPSC-derived ventricular-like cardiomyocytes

Ventricular cardiomyocytes generated with our standardized process are fully functional. Their relatively slow beat rate enables pacing for electrophysiological research. Base on this model, an array of functional assays can be developed and optimized providing relevant predictions of drug-induced responses.

Our team can generate cardiomyocytes from the iPSC that best fits your project or work with off-the-shelf Ncyte® vCardiomyocytes to support your projects.

cardiac
Immunofluorescence staining of Ncyte® vCardiomyocytes. Cardiac Troponin T (Green) and DAPI (Blue).
Benefits
  • High purity ventricular-like cardiomyocytes
  • Physiologically relevant
  • Highly suitable for electrophysiological research

Do you want to buy Ncyte® vCardiomyocytes?

Do you need a custom cell model?

Product Specifications

Identity markers

≥ 70% TNNT2 at day 3 of culture according user guide

Size

≥ 4M viable cells at day 3 of culture according to user guide

Quality Control

Cell count, Viability, Identity (FACS), Functionality (MEA), Mycoplasma


 

 
Format

Cryopreserved cells
 
Donor

Female
 
Reprogramming method

Non-viral
 

 

 
Shipping conditions

Dry shipper, -180°C to -135°C
 
Storage conditions

Vapor phase of liquid nitrogen

Supplied with

Ncyte® Cardiomyocyte Medium

Technical Data

Ncyte® vCardiomyocytes are characterized by flow cytometry to ensure a purity of ≥ 70% cardiac TroponinT (cTnT) positive cells after 3 days in culture following Ncardia’s user guide (Figure 2A). They typically express Myosin Light Chain 2v (MLC2v), indicating a ventricular-like phenotype (Figure 2B). Based on RNA-seq data, Ncyte® vCardiomyocytes express common sarcomere markers, such as TNNT2, while hiPSC and cardiac progenitor markers are silenced. Expression levels of additional genes are available upon request.

A) Flow cytometry analysis of one representative batch showing cTnT positive cells (99%) and cTnT/MLC2v double positive cells (76%). B) Histograms of cTNT and MLC2v fluorescence signal and their antibody controls.

Ncyte® vCardiomyocytes exhibit well aligned myofibrils and intact structural sarcomere organization (See figure A). They typically express Myosin Light Chain 2v (MLC2v), indicating a ventricularlike phenotype. Based on RNA-seq data, Ncyte® vCardiomyocytes express common sarcomere markers, such as TNNT2, while hiPSC and cardiac progenitor markers are silenced. Expression levels of additional genes are available upon request.

Their action potential shows fast upstroke velocity and a well-defined plateau phase, resembling human ventricular cardiomyocytes (See figure B).

These make the Ncyte® vCardiomyocytes a physiologically relevant model for the phenotypic study of cardiac diseases. Our expert scientists can develop and execute predictive assays to support your drug discovery research in the cardiac field.

Ncyte® vCardiomyocytes present a relatively slow and uniform beating rate, which enables pacing for electrophysiological research (See top figure). Additionally, response to adrenergic stimulation with isoproterenol and ion channel blockers, nifedipine (L-type calcium channel blocker) and dofetilide (hERG channel blocker), is guaranteed for every batch (See figure A, B, C). Our scientific team is experienced in the electrophysiological analysis of Ncyte® vCardiomyocytes for safety and efficacy studies and can report the analyzed results to you.

 

A) 30-minute exposure to isoproterenol induced an increase in beat rate of Ncyte® vCardiomyocytes. B) 30-min exposure to Dofetilide, a hERG (IKr) channel blocker, induced a dose dependent prolongation of field potential duration as well as notable arrhythmias in Ncyte® vCardiomyocytes, at concentrations of ≥10 nM. C) 30-minute exposure to Nifedipine, an L-type calcium channel blocker, shortened the field potential duration of Ncyte® vCardiomyocytes in a dosedependent manner. Data presented as mean ±SD (n=6) of the % change to baseline recordings for each well and time-matched 0.1% DMSO control.