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Ncyte® Endothelial Cells

Human iPSC-derived vascular endothelial cells

Endothelial cells are involved in multiple processes including blood flow, vascular permeability and angiogenesis. Ncyte® Endothelial Cells are fully functional and can be used to develop custom assays to predict drug-induced effects on human endothelial barrier permeability or angiogenesis.

You can work with Ncyte® Endothelial Cells or contact our team to develop a controlled and scalable process for endothelial cells from the iPSC line that fits your project best.

 

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Ncyte® Endothelial Cells in culture
Benefits
  • Minimal biological variability
  • Functional and physiologically relevant
  • Highly suitable for in vitro modeling and screening

Do you want to buy Ncyte® endothelial cells?

Do you need a custom cell model?

Product Specifications

Identity markers

≥ 70% CD31, ≥ 70% CD144, ≤ 10% PDGFRB and ≤ 10% NG2 on Day 0 and Day 3 after thawing and culture according to the user guide

Size

≥ 1M viable cells on Day 0 after thawing according to user guide

Quality Control

Cell count, Viability, Identity (FACS), Specific-pathogen-free

 

Format

Cryopreserved cells
 
Donor

Female
 
Reprogramming method

Non-viral

 

Shipping conditions

Dry shipper, -180°C to -135°C
 
Storage conditions

Vapor phase of liquid nitrogen

Technical Data

Ncyte® Endothelial Cells display characteristic endothelial cell morphology in culture and express key endothelial markers. As an example, panel one contains a representative FACS analysis of one batch of Ncyte® Endothelial Cells showing double expression of CD144 and CD31. Second panel is a phase contrast image showing endothelial morphology in culture.

Ncyte® Endothelial Cells are fully functional and physiologically relevant. The cells can form endothelial barriers in vitro, and our scientists can assess drug-induced effects on endothelial barrier permeability and angiogenesis with this iPSC-derived cell model.

The figure shows barrier resistance measured at 4 kHz over time using ECISTM (Applied Biophysics).

Ncyte® Endothelial Cells are highly suitable for disease modeling and screening. Our scientific team has extensive experience on multiple assays. As an example, our team can help you determine the effect of a compound library on angiogenesis using the sprouting assay.

Dose dependent inhibition of angiogenic sprouting by the angiogenesis inhibitor Sunitinib