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Ncyte® Excitatory Neurons

Human iPSC-derived excitatory glutamatergic neurons

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Ncyte® Excitatory Neurons are human iPSC-derived glutamatergic neurons that develop characteristic neuronal morphology and express key markers of excitatory neuronal identity.

Designed for CNS drug discovery and safety research, Ncyte® Excitatory Neurons provide a human-relevant model for investigating excitatory signalling, synaptic function and neuronal network biology, with applications in areas including seizure liability, excitotoxicity and synaptic dysfunction.

Benefits
  • Characterised excitatory glutamatergic neuronal population
  • Suitable for synaptic and neuronal network studies
  • Applicable to CNS drug discovery and safety research
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Immonofluorescence staining of Ncyte (R) Excitatory neurons. vGLUT1 (Green), MAP2 (Green), DAPI (Blue)

 
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Looking for a kit that fits your workflow?

Choose from Day 7, Day 14, or Day 28 Ncyte® Neuron Kits, providing the cells and culture components needed for your selected culture period

Product Specifications

Identity markers

TUBB3 and vGLUT1 at day 7 post-thaw, according to the user guide.

Size

≥1 million viable cells at thawing.

Quality Control

Cell count, Viability, Identity, Mycoplasma

Format

Cryopreserved cells

Donor

Female

Reprogramming method

Ectopic expression of reprogramming factors using episomal vector

Shipping conditions

Dry shipper, -180°C to -135°C, supplements on dry ice

Storage conditions

Vapour phase of liquid nitrogen

Supplied with

Required supplements and media for initial culture according to the Ncyte® Excitatory Neurons User Guide.

Technical Data

Cellular Identity Physiologically Relevant Highly suitable for drug discovery

Ncyte® Excitatory Neurons develop characteristic neuronal morphology and extensive neurite growth following thawing and culture.

Excitatory neuronal identity is demonstrated by expression of the pan-neuronal marker TUBB3 and the glutamatergic neuron marker vGLUT1.

Differentiated neurons can be observed from Day 1–2 post-thaw. For more mature neuronal morphology, culture until at least Day 7 is recommended.

Immunofluorescence staining of Ncyte® Excitatory Neurons at Day 7 post-thaw demonstrates expression of TUBB3 and vGLUT1.

Excitatory neurons are an important component of neuronal networks and provide a relevant human cellular system for studying synaptic signalling and network activity.

Ncardia is evaluating Ncyte® Excitatory Neurons in functional neuronal assays, including multi-electrode array measurements. In internal studies, excitatory neurons cultured with astrocytes developed measurable network activity and met predefined MEA performance criteria by Day 21.

This work supports the potential use of Ncyte® Excitatory Neurons in functional CNS research where neuronal firing and network behavior are relevant experimental readouts.

Ncyte® Excitatory Neurons are well suited for electrophysiological studies, neuronal network research and disease modelling where altered excitatory signalling and synaptic function play an important role. They can support applications including seizure liability, neurotoxicity and the investigation of mechanisms associated with CNS diseases. Our neuroscience team can support assay development and functional screening, helping you assess the efficacy and safety of therapeutic candidates in human-relevant neuronal models.

 

Product Information and Applications

Certificates of analysis are available upon request via support@ncardia.com

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Questions about our cell models?

Our work centers on a simple premise: combining deep iPSC knowledge, broad assay capabilities, and human-relevant cellular biology can help drug developers answer critical questions earlier in development. Talk to our scientific team about how Ncyte® Excitatory Neurons could fit your CNS discovery or safety programme.