Skip to content
HOME cell supply ncyte cholinergic neurons

Ncyte® Cholinergic Neurons

Human iPSC-derived cholinergic neurons

Order Now

Ncyte® Cholinergic Neurons are human iPSC-derived neurons that develop characteristic neuronal morphology and express key markers of cholinergic neuronal identity.

Designed for CNS drug discovery and disease research, Ncyte® Cholinergic Neurons provide a human-relevant model for studying cholinergic biology and mechanisms relevant to cognition, memory and neurodegenerative diseases such as Alzheimer’s disease.

Benefits
  • Characterised excitatory glutamatergic neuronal population
  • Relevant for cognition and neurodegeneration research
  • Suitable for pharmacology, neurotoxicity, and screening in a human neuronal model
neural

Immunofluorescent staining of cholinergic neurons on Day 3 post-thaw shows expression of TUBB3 and the cholinergic neuron marker ChAT.

 
Do you want to buy Ncyte® Cholinergic Neurons?
Accept Our Terms to Order

To proceed with your order, please review and accept our Terms and Conditions above.

 

 

 

Looking for a kit that fits your workflow?

Choose from Day 7, Day 14, or Day 28 Ncyte® Neuron Kits, providing the cells and culture components needed for your selected culture period

Product Specifications

Identity markers

TUBB3 and ChAT

Size

Approximately 1 × 10⁶ viable cells per vial.

Quality Control

Cell count, Viability, Identity, Mycoplasma

Format

Cryopreserved cells

Donor

Female

Reprogramming method

Ectopic expression of reprogramming factors using episomal vector

Shipping conditions

Dry shipper, -180°C to -135°C, supplements on dry ice

Storage conditions

Vapour phase of liquid nitrogen

Supplied with

Required supplements and media for initial culture according to the Ncyte® Cholonergic Neurons User Guide.

Technical Data

Cellular Identity Physiologically Relevant Highly suitable for drug discovery

Ncyte® Cholinergic Neurons display characteristic neuronal morphology and develop extensive neurite networks in culture. They express the pan-neuronal marker TUBB3 and the cholinergic neuron marker ChAT, supporting their cholinergic neuronal identity.

Immunofluorescence staining of Ncyte® Cholinergic Neurons at Day 7 post-thaw demonstrates expression of TUBB3 and vGLUT1.

Cholinergic neurons are a physiologically relevant tool for in vitro studies of human cholinergic signalling and neuronal biology. Cholinergic signalling plays an important role in cognitive processes including learning and memory and is particularly relevant to the study of neurodegenerative diseases such as Alzheimer’s disease.

Ncyte® Cholinergic Neurons provide a human neuronal model for investigating cholinergic biology and can support the development of physiologically relevant CNS research models.

Ncyte® Cholinergic Neurons are highly suitable for drug discovery and disease modelling studies where altered cholinergic function plays an important role, including Alzheimer’s disease and other disorders affecting cognition and memory. They can also support cholinergic pharmacology, neurotoxicity studies and the evaluation of therapeutic candidates targeting cholinergic pathways. Our team of neuroscientists is experienced in assay development and screening and can help you assess the efficacy and safety of therapeutic candidates in human-relevant neuronal models.

Product Information and Applications

Lot Information Sheet is available upon request via support@ncardia.com

Related cell models
Questions about our cell models?

Our work centers on a simple premise: combining deep iPSC knowledge, broad assay capabilities, and human-relevant cellular biology can help drug developers answer critical questions earlier in development. Talk to our scientific team about how Ncyte® Excitatory Neurons could fit your CNS discovery or safety programme.