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Ncyte® Neural Mix

Co-culture of human iPSC-derived neurons and astrocytes

The cells are simultaneously generated from neural precursors following our standardized procedure. This model recapitulates the interplay between excitatory and inhibitory neurons. Their rapid formation of neuronal networks and prolonged viability in culture, make this model ideal for early drug discovery projects. Note that this product was formerly known as Ncyte® CNS Neurons. 

Our team can generate neurons and astrocytes from the iPSC that best fits your project or work with Ncyte® Neural Mix.

neural
TH (red), βIII-Tubulin (Green) & DAPI (blue)
Benefits
  • Physiologically relevant neural networks
  • Fast maturation process
  • Long-term synchronous network activity

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Product Specifications

Identity markers

≥ 50% βIII Tubulin,  ≤ 50% GFAP, ≥ 5% GFAP/Sox1,  ≤ 20% KI67 cells at day 14 post thaw according to user guide

Size

≥ 2M viable cells after thawing according to user guide

Quality Control

Cell count, Viability, Identity (FACS), Functionality (MEA), Mycoplasma

 

Format

Cryopreserved cells
 
Donor

Female
 
Reprogramming method

Non-viral

 

Shipping conditions

Dry shipper, -180°C to -135°C
 
Storage conditions

Vapor phase of liquid nitrogen

Supplied with

Ncyte® Neuronal Supplement

Technical Data

The Ncyte® Neural Mix has in its composition glutamatergic neurons, identified by the expression of the presynaptic vesicular glutamate transporter vGlut1 and dopaminergic neurons, identified by the expression of the TH marker. It also contains astrocytes that express the astrocyte-specific marker GFAP.

Ncyte® Neural Mix cells are fully functional human cells which form neuronal networks in vitro and enable short and long-term electrophysiological studies. As an example, they show robust spontaneous activity on multi-electrode array (MEA) systems.

 

Ncyte® Neural Mix is highly suitable for disease modeling of neurodegenerative disease and epilepsy, offering a sensitive response to compounds. Our scientific team has extensive experience on developing and optimizing assays to study electrophysiology or drug-induced neurotoxicity, among other parameters.

Ncyte® Neural Mix shows specific response to GABA-A blocker picrotoxin at 3uM, demonstrating the presence of functional GABA-A receptors.